High universality of matK primers for barcoding gymnosperms
نویسندگان
چکیده
منابع مشابه
Universal multiplexable matK primers for DNA barcoding of angiosperms1
PREMISE OF THE STUDY PCR amplification of the matK barcoding region is often difficult when dealing with multiple angiosperm families. We developed a primer cocktail to amplify this region efficiently across angiosperm diversity. METHODS AND RESULTS We developed 14 matK primers (seven forward, seven reverse) for multiplex PCR, using sequences available in GenBank for 178 taxa belonging to 123...
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MaturaseK gene (MatK) of chloroplast is highly conserved in plant systematics which is involved in Group II intron splicing. The size of the gene is 1500 bp in length, located with in the intron of trnK. In the present study, matK gene from Zingiberaceae was taken for the analysis of variants, parsimony site, patterns, transition/tranversion rates and phylogeny. The family of Zingiberaceae comp...
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ژورنال
عنوان ژورنال: Journal of Systematics and Evolution
سال: 2011
ISSN: 1674-4918
DOI: 10.1111/j.1759-6831.2011.00128.x